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Bulletin of the World Health Organization : 1983; Volume 61, Number 5, Year 1983 61 (5), Pages 745-755: Nutritional Surveillance

By World Health Organization

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Book Id: WPLBN0000000244
Format Type: PDF eBook
File Size: 0.5 MB
Reproduction Date: 2005

Title: Bulletin of the World Health Organization : 1983; Volume 61, Number 5, Year 1983 61 (5), Pages 745-755: Nutritional Surveillance  
Author: World Health Organization
Volume:
Language: English
Subject: Health., Public health, Wellness programs
Collections: Medical Library Collection, World Health Collection
Historic
Publication Date:
Publisher: World Health Organization

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Organization, W. H. (n.d.). Bulletin of the World Health Organization : 1983; Volume 61, Number 5, Year 1983 61 (5), Pages 745-755. Retrieved from http://worldebooklibrary.com/


Description
Medical Reference Publication

Excerpt
Val.iola virus was irle~ltifierbl y means of n ,,ietlzod invol>,i,rg the fijr~~zationo f microfoci on lnicroplate cultures of HeLa cells. If ivrrs po.wible to rlisti~~grrish between vai.io1rr nrnjor and variola r~rinor vimses tvitlti,~4 8 h of rgceipt ofrlie specimens by rlcrermining the ?e,~i~erat~serrrres ilir,ity of fucris formation. A rapid method for the diagnosis of smallpox in the laboratory is required in countries where the disease is not endemic in order to determine the immediate public health action to be taken. The current routine laboratory diagnostic procedures for differentiating variola virus from other poxviruses by pock morphology on chorioallantoic membranes (CAM) and for differentiating between variola major and minor viruses by the ceiling temperatures of pock formation (Dumbell et al., 1961; Durnbell, 1968) have several limitations. First, the techniques require a readily available supply of fertile egss of the correct incubation ase (11-13 days) and a large amount of incubator space at temperatures differing by as little as 02°C. Secondly. pock morpholosy cannot be used as an absolute criterion in view- of the fact that monkeypox virus forms pocks apperently indistinguishable from those produced b) ~ariola viruses (Lourie et al., 1972). 111 ~irr-ud etection and quantification of ~ariola w:ui by. '' h>rcrplasric focus formation in human ccll cultures has been reported (Pirsch & Purlson, 1962; Kiramura, 1968) and the technique has been appi~ed successfully to the quantitative neutralizat~on rest nirh poxvims antibodies (Kitamura & Shinjo, 1972). The p;ocedure has been modified so that it can be carried our in the flat-hottonled wells oE plastic Microtiter platei.Wne such plate \viith 95 wells of 6 mm diarnerer each

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